eng
Scientific Society for Flowers and Ornamental Plants
Scientific Journal of Flowers and Ornamental Plants
2356-7864
2536-9296
2018-09-01
5
3
219
231
10.21608/sjfop.2018.24212
24212
Original Research Article
IN VITRO CULTURE AND STUDYING THE CHEMICAL COMPOSITION OF THE ESSENTIAL OILS EXTRACTED FROM THREE SAMPLES OF ERIOCEPHALUS AFRICANUS L. PLANT IN EGYPT
T.A.D. Mohamed
1
A.M.A. Habib
2
M.M. EL-Zefzafy
3
A.I.E. Soliman
4
Ornamental Horticulture Dept., Fac. Agric., Cairo Univ., Egypt
Ornamental Horticulture Dept., Fac. Agric., Cairo Univ., Egypt
Medicinal Plants (Plant Tissue Culture) Dept., National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Medicinal Plants (Plant Tissue Culture) Dept., National Organization for Drug Control and Research (NODCAR), Giza, Egypt
The present study aimed to establish new protocol for propagation via tissue culture techniques to observe the effect of plant growth regulators especially cytokinins, gibberellic acid and auxins with different concentrations on in vitro growth of Eriocephalus africanus L. for improving the potentiality of regeneration and secondary metabolites production and identification of the main active constituents of volatile oil by GC/MS. The results showed that, the best sterilization treatment was the shoot tip explants rinsed in a solution of clorox at 15% for 15 min was gave the highest values for survival percentage and plant strength 100% and 4.58, respectively also B5 medium at full strength gave the best results in the both growth measurements. BAP at 2.00 mg/l recorded the highest values in survival percentage (93.33%), shootlet number/cluster (16.50) and shootlet strength (4.50), respectively. Using the high level from GA3 (4.00 mg/l) in medium was more effective in the elongation of shootlets. In rooting stage B5 medium supplemented with 0.50 mg/l IBA and 0.15% active charcoal was more effective for increasing root number/explant to 8.67 and root length to 5.78 cm. The chemical analysis for the volatile oils extracted from three samples (field, in vivo and in vitro) of Eriocephalus africanus L. by using GC/MS confirmed that, the total number of the constituents identified ranged from 30-34 compounds representing 79.67-99.41% of the total oil contents. Artemisia ketone (17.10-30.62%), bicyclogermacrene (4.14-15.56%), globulol (2.17-8.30%), allo-aromadendrene epoxide (0.51-9.00%), caryophyllene oxide (2.85-6.76%) and α-pinene (3.10-4.25%) were the main compounds.
https://sjfop.journals.ekb.eg/article_24212_6c6758f9acb29c746ea21d78047b0ba4.pdf
Eriocephalus africanus L
B5 medium
BAP
GA3
IBA
Active Charcoal
Volatile oils
GC-MS and Artemisia ketone
eng
Scientific Society for Flowers and Ornamental Plants
Scientific Journal of Flowers and Ornamental Plants
2356-7864
2536-9296
2018-09-01
5
3
233
243
10.21608/sjfop.2018.24216
24216
Original Research Article
INVESTIGATION OF MORPHOLOGICAL AND ANATOMICAL CHANGES IN CATHARANTHUS ROSEUS (L.) G. DON DUE TO COLCHICINE INDUCED POLYPLOIDY
Awad Shala
awad.shala@yahoo.com
1
Z. Deng
zdeng@ufl.edu
2
Medicinal and Aromatic Plants Research Department, Sakha Horticulture Research Station, Horticulture Research Institute. Agricultural Research center.
Department of Environmental Horticulture, Gulf Coast Research and Education Center, University of Florida, USA.
Catharanthus roseus (L.) G. Don is valuable medicinal plant grown as an ornamental plant. The study was carried out at Gulf Coast Research and Education Center, University of Florida, USA. to evaluate the morphological and anatomical changes from induced polyploidy in periwinkle for this purpose colchicine was applied at four different concentrations 0.0, 0.05, 0.1 and 0.2% for seedling treatment. The results showed that raising colchicine doses caused an increasing of survival percentage and tetraploid plants number. Polyploids showed an increment in leaf thickness, stomatal size, pollen diameter and pollen viability compared to diploid plants. Flow cytometry verified the existence of tetraploids and mixoploids from various colchicine-treated plants especially 0.2 which was found efficient on inducing polyploidy in the plants followed by 0.1% as compared to other concentration under study.
https://sjfop.journals.ekb.eg/article_24216_42e098fff216aa79bd31c09b1de59f1c.pdf
Vinca
chromosome doubling
Colchicine
nuclear DNA content
tetraploid
eng
Scientific Society for Flowers and Ornamental Plants
Scientific Journal of Flowers and Ornamental Plants
2356-7864
2536-9296
2018-09-01
5
3
245
259
10.21608/sjfop.2018.26604
26604
Original Research Article
RESPONSE OF JATROPHA CURCAS L. SHRUBS TO COMPLETE (NPK) FERTILIZER, BIO AND ORGANIC FERTILIZERS UNDER SANDY SOIL CONDITIONS
E. El-Azzony
1
E. El-Mogy
2
N. El-Shaip
3
Medicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Giza, Egypt.
Medicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Giza, Egypt.
Medicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Giza, Egypt.
The present work was carried out during the two successive seasons of 2015 and 2016 at the Experimental Farm of El-Quassassin Horticultural Research Station, Ismailia Governorate, Egypt, to study the effect of complete NPK fertilization rates (control, 50, 75 and 100% from the recommended dose), bio and organic fertilizers (control, EM1 applied as the rate of 100 ml/shrub and active dry yeast with the concentration of 4 g/las a rate of 2 liters/shrub) and the interaction treatments between them on the growth, (fruits and seeds yields), fixed oil content and its components in seeds of Jatropha (Jatropha curcas) shrubs.The results showed that, the best vegetative growth parameters (increasing percentage of shrub height, number of branches and canopy width/shrub), number and weight of fruits (kg)/shrub, number and weight of seeds (kg)/shrub, the yield of fruits and seeds (ton)/fed, fixed oil percentage, weight of fixed oil (g)/shrub and yield of fixed oil (kg)/fed were recorded from the interaction treatment of 75% complete NPK fertilization + EM1. The analysis of fixed oil of Jatropha shrub showed that, the treatment of 50% NPK gave the best palmitic acid percentage and the treatment of 50% NPK + EM1 recorded maximum percentages of stearic and oleic acids, while the treatment of 100% NPK + EM1 gave the highest percentage of linoleic acid.
https://sjfop.journals.ekb.eg/article_26604_8e63505bf16b8033490c2452a83a43ef.pdf
Jatropha curcas
NPK fertilizer
EM1
Active dry yeast
fixed oil
eng
Scientific Society for Flowers and Ornamental Plants
Scientific Journal of Flowers and Ornamental Plants
2356-7864
2536-9296
2018-09-01
5
3
261
273
10.21608/sjfop.2018.31467
31467
Original Research Article
RESPONSE OF TUBEROSE PLANTS (POLIANTHES TUBEROSA L.) TO CHEMICAL AND BIO FERTILIZATION AND THEIR EFFECT ON VEGETATIVE GROWTH, FLOWERING AND CHEMICAL COMPOSITION UNDER SANDY SOIL CONDITIONS
K.E. Attia
1
Naglaa Elbohy
2
Nahla Ashour
3
Medicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Egypt.
Ornamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Egypt.
Medicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Egypt.
A field experiment was carried out at the Experimental Farm of El-Kassasin Horticultural Research Station, Agricultural Research Center, Ismailia Governorate, Egypt, to study the effect of partial substitution of chemical nitrogen fertilizer with biofertilizer containing two strains of nitrogen-fixing (Azospirillum lipoferum, Azotobacter chrococcoum), on vegetative growth, flowering and chemical composition of tuberose plants (Polianthes tuberosa L.) under sandy soil conditions to rationalize the use of mineral fertilizers and reduce pollution of the environment in order to increase the quantity and improvement quality of the product on the tuberose plants during the two successive seasons of 2017 and 2018. This experiment included seven treatments with three replications, which were the combinations between two of mineral nitrogen rates (50% and 75%) and three bio-fertilizers of nitrogen-fixing bacteria (Azospirillum lipoferum (S), Azotobacter chrococcoum (Z) and Azospirillum lipoferum + Azotobacter chrococcoum (S*Z) treatments in addition to the control which was 100% N. The treatments were arranged in Randomized Complete Block Design. The results showed that fertilizing tuberose plants with mineral nitrogen at 100% of the recommended dose recorded the highest values for plant height, number of leaves/plant and number of florets/spike. while fertilization with mineral nitrogen at 75% plus the dual inoculation with Azospirillum + Azotobacter recorded the highest values for fresh and dry weights of leaves (g)/plant, fresh and dry weights of root (g)/plant, fresh and dry weights of spike (g)/plant, floret diameter (cm), spike length (cm), longevity of tuberose cut flower spikes (days), in addition to leaves content of N, P, K, total chlorophyll a, b and carbohydrates %, as well as essential oil percentage in flowers without significant difference between them and control in most cases in both seasons.
https://sjfop.journals.ekb.eg/article_31467_e56270c12601811fa5921f9ea7b997e4.pdf
Tuberose
spike
nitrogen
Bio-fertilizers
Azospirillum and Azotobacter