Scientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331STUDIES ON SWEET BASIL MOSAIC VIRUS DISEASE AND ITS CONTROL BY USING SOME ESSENTIAL OILS1131281310.21608/sjfop.2018.12813ENF. E.M.SalehMedicinal and Aromatic Plants Research Department, Horticulture Research Institute,
Agricultural Research Center (ARC), Giza, EgyptA. A.S.Abdel-KaderMedicinal and Aromatic Plants Research Department, Horticulture Research Institute,
Agricultural Research Center (ARC), Giza, EgyptAmal M.I.ErakyDepartment of Plant Pathology, Faculty of Agriculture, Assuit University, EgyptJournal Article20180303The current study, the mosaic virus disease of <em>Ocimum basilicum</em> L. (sweet basil) was identified and the different effects of three essential oils on growth, oil content and composition as well as reducing CMV disease symptoms of sweet basil plants <em>in vivo</em>. During 2012 season, natural occurrence of mosaic virus disease was observed on sweet basil in Assiut, Egypt. The causal agent of basil mosaic disease was detected as cucumber mosaic virus (CMV) by PCR product that revealed presence of approximately expected size (~ 781 bp) in naturally infected basil. Therefore, a field experiment was carried out during two successive seasons 2013 and 2014 on sweet basil plants at the Experimental Farm of Arab El Awamer Agricultural Research Station, Assiut Governorate, Egypt. Ten treatments of three essential oils extracted from lemongrass (<em>Cymbopogon citratus</em>), rosemary (<em>Rosemarinus officinalis</em>) and peppermint (<em>Mentha piperita </em>L<em>.</em>) plants were applied as foliar spray at 1% concentration before and after inoculation with isolated virus and two controls (healthy and infected plants) were arranged in a randomized complete block design with three replicates. Results revealed that application of essential oils under field condition improved growth and yield (branches, weight of fresh and dry plant (g/plant) as well as essential oil percentage), reduced the CMV disease symptoms compared to untreated infected control. As well as significant increase in total identified of essential oil composition of basil plant. Whereas the treatment with rosemary oil before and after inoculation respectively, was increased in myrcene, linalool when applied before inoculation and with a great increase in 1, 8-cineole when applied after inoculation. Also a great increase was related to peppermint oil treatment for eugenol, myrcene and 1, 8-cineole % before inoculation comparable to untreated infected plants. While, using of peppermint oil significantlyincreased number of branches, fresh and dry weights, essential oil content and composition when applied before inoculation with virus compared to infected and healthy controls. Lemongrass essential oils exhibited a moderate protective effects. Generally, applying of rosemary essential oil as curative seemed to be superior for improving branches, weight of fresh and dry plant (g/plant), and essential oil percentage and composition comparing to other treatments.https://sjfop.journals.ekb.eg/article_12813_bcaed094d5272eba4e65e566597e550c.pdfScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331SUPPRESSION OF DAMPING-OFF DISEASE BY SOME MICROORGANISMS ON SWEET BASIL (OCIMUM BASILICUM L.) PLANTS UNDER FIELD CONDITIONS15291281410.21608/sjfop.2018.12814ENHowida A.MetwalyCentral Lab. of Organic Agriculture, Agricultural Research Center, Giza, EgyptM. A.Abd-El-SayedMedicinal and Aromatic Plant Research Department, Horticulture Research Institute, Agricultural Research Center, Giza, EgyptJournal Article20180305This study was carried out at the Experimental Farm of Medicinal and Aromatic Plants Research Department in El Kanater El Khairia, HRI, ARC, during the two successive seasons of 2016 and 2017 to study the effect of some microorganisms (<em>Bacillus subtilis</em>,<em> Trichoderma</em> <em>harzianum</em>,<em> Pseudomonas fluoroscens</em> and<em>Streptomyces griseus</em>) on suppressing of damping-off disease of basil seedling, vegetative growth, oil production and chemical constituents of sweet basil (<em>Ocimum basilicum</em> L.) plants. The plants treated with any of the bioagents, showed highest effect in controlling damping-off and increased basil productivity compared with control plans. The results showed that, all bioagents treatments significantly increased vegetative growth (plant height, number of branches, fresh and dry weights (g/plant), oil percentage, oil yield, chemical constituents (chlorophyll (a), (b) and carotenoids contents as well as total carbohydrates percentage) compared with control plants. <em>Streptomyces griseus </em>gave the best effect against damping-off disease and all plant characteristics compared with control plants in the first season while, in the second season,<em> Pseudomonas fluorescens</em> increased plant protection and all plant characteristics. GC analysis of sweet basil essential oil of all treatments identified nine components. The major component of the essential oils was Linalool.https://sjfop.journals.ekb.eg/article_12814_ab6f60daf7a2c8aa12f2621bc8070c7e.pdfScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331EFFECT OF SOME POST-HARVEST TREATMENTS ON CUT LEAVES OF CHAMAEDOREA ELEGANS31441281510.21608/sjfop.2018.12815ENY. M.E.El-ShewaikhOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptEhsan E.A.El-DeebOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptZeinab H.El-SadekOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptJournal Article20180308This study was carried out at the Post-harvest Lab. of Ornamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., Giza, Egypt during the two seasons of 2014/2015 and 2015/2016. The aim of this study is to investigate the effect of GA<sub>3</sub>, BA, 8-HQC, CA and Sug. as preservative solution on improving the quality of the leaves, delaying leaf wilting and leaf yellowing and extending the shelf life period of cut leaves of <em>Chamaedorea elegans</em>. The results showed that, the treatment of the solution containing T14: GA<sub>3</sub> at 50 ppm + BA at 20 ppm + 8-HQC at 300 ppm + CA at 300 ppm + Sug. at 2%, followed by the solution containing the T13: GA<sub>3</sub> at 50 ppm + BA at 20 ppm + CA at 300 ppm + Sug. at 2% significantly increased the water uptake, water balance, general appearance, vase life, concentration of chlorophyll a, b, carotenoids and total carbohydrates. It also reduced the amount of water loss in all days compared to other treatments. It can be recommended holding cut foliage of <em>Chamaedorea elegans </em>in solution containing GA<sub>3</sub> at 50 ppm + BA at 20 ppm + 8-HQC at 300 ppm + CA at 300 ppm + Sug. at 2% followed by the solution containing GA<sub>3</sub> at 50 ppm + BA at 20 ppm + CA at 300 ppm + Sug. at 2% to improve cut leaves quality and longer vase life was reached 84 days.https://sjfop.journals.ekb.eg/article_12815_076d947900e42f5f51baf9a582314841.pdfScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331EFFECT OF SOME POST-HARVEST TREATMENTS ON UNROOTED CUTTING OF DRACAENA MARGINATA45561281610.21608/sjfop.2018.12816ENEhsan E.A.El-DeebOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptZeinab H.El-SadekOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptY. M.E. El-ShewaikhOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptJournal Article20180308This study was carried out at the Post-harvest Lab. of Ornamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., Giza, Egypt during the two seasons of 2014/2015 and 2015/2016. The aim of this study is to investigate the effect of GA<sub>3</sub>, BA, 8-HQC, CA and Sug. as the preservative solution to improve the quality of the unrooted cutting, delaying leaf wilting, leaf yellowing and extending the shelf life period of unrooted cutting of <em>Dracaena marginata</em>. The results revealed that, the treatment of the solution containing the GA<sub>3</sub> at 50 ppm + BA at 20 ppm + 8-HQC at 300 ppm + CA at 300 ppm + Sug. at 2%, followed by the solution containing GA<sub>3</sub> at 50 ppm + BA at 20 ppm + CA at 300 ppm + Sug. at 2% significantly increased the water uptake, water balance, general appearance, vase life, chlorophyll a, b, carotenoids and total carbohydrates. It also reduced the amount of water loss in all days compared to other treatments. It can be recommended that holding unrooted cutting of <em>Dracaena marginata </em>in a solution containing GA<sub>3</sub> at 50 ppm + BA at 20 ppm + 8-HQC at 300 ppm + CA at 300 ppm + Sug. at 2%, followed by the solution containing GA<sub>3</sub> at 50 ppm + BA at 20 ppm + CA at 300 ppm + Sug. at 2% to improve quality and giving longer vase life was reached 50 days.https://sjfop.journals.ekb.eg/article_12816_bc96629fdbe1242bc0dc1fa1873eaef5.pdfScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331IN VITRO MULTIPLICATION OF VANGUERIA EDULIS AS AFFECTED BY CYTOKININS AND MEDIUM TYPE57651281810.21608/sjfop.2018.12818ENH. M.A.El-BagouryOrnamental Horticulture Dept., Fac. Agric., Cairo Univ., EgyptA. M.Z.SarhanOrnamental Horticulture Dept., Fac. Agric., Cairo Univ., EgyptF. M.SaadawyOrnamental Plants & Landscape Gardening Res. Dept., Hort. Res. Inst., Agric. Res. Cent., Giza, EgyptMai M.M.EbrahimOrnamental Plants & Landscape Gardening Res. Dept., Hort. Res. Inst., Agric. Res. Cent., Giza, EgyptJournal Article20180227This work was carried out in the Tissue Culture Laboratory, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt during the period from 2015 to 2017, to investigate the effect of cytokinin type and concentration (BAP, 2-iP and kin, at either 0.0, 1.0, 2.0 and 3.0 ppm) as well as medium type (MS or B5) on micro-shoots multiplication of <em>in vitro</em> cultured rare ornamental plant, <em>Vangueria edulis</em>.<br /> Results that were significant could be briefed in the following: the first position was obtained by BAP at 1 ppm in regard to survival %, shoot length and shooting %; BAP at 3 ppm for shoot number, leaf number and shoot length; 2-iP at 1 ppm for shooting %; 2-iP at 2 ppm for shoot length and shooting %; and kinetin at 2 ppm for survival %.<br /> Using MS medium gave rise to higher values concerning survival %, shoot number, leaf number, shoot length and shooting % compared to using B5 medium.<br /> The highest position was occupied by the combinations between BAP 1 ppm + MS for survival %, shoot number, shoot length and, shooting %; BAP 2 ppm + MS for shoot number; BAP 3 ppm+B5 for shoot number and leaf number; 2-iP 1 ppm + MS for shooting %; 2-iP 2 ppm + MS for shoot number; kinetin at 1 or 3 ppm + MS for shoot length and kinetin 2 ppm + MS for survival % and shoot length.<br /> It is recommended to treat <em>in vitro</em> produced micro-shoots of <em>Vangueria edulis</em> with BAP at 1 ppm + MS medium to obtain the highest values during multiplication stage.https://sjfop.journals.ekb.eg/article_12818_fdb6141093f4e6f0efd55151b86f3e00.pdfScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331ECONOMICAL STUDY OF IN VIVO AND IN VITRO PROPAGATION OF RUSSELIA EQUISETIFORMIS67871281910.21608/sjfop.2018.12819ENM. S.MeshaalDepartment of Agricultural Economics, Fac. Agric., Cairo Univ., Giza. EgyptK. A.EmamBotanical Garden Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptF. Z.GharebBotanical Garden Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptE. A.KhellaOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Giza, EgyptJournal Article20180225This experimental trial was performed throughout two successive seasons (2015-2016) and (2016-2017) at the nursery of Horticulture Research Institute, Agricultural Research Center, Giza, Egypt. Intended to find out the effect of the individual and combined effects of different growing media with different types of propagation on morphological traits and some chemical constituents of <em>Russelia equisetiformis </em>Schlecht. & Cham plant. Accordingly, on September 1<sup>st</sup>, in the two seasons, different plant parts of the whole plant (tip and mid cutting of 10-12 cm length, besides divisions containing foliage and root parts were planted in 10 cm plastic pots (one plant/pot) for every growing media used. The plants were left to grow under open field conditions. The layout of the experiment was a factorial experiment in randomized complete block design (RCBD) with three replicates (2 growing media × 3 types of propagation). The results emphasized that the mixture of sand + 15% compost proved its mastery in improving vegetative growth parameters (plant height, number of branches/plant, fresh and dry weight of shoots). Besides, it was the best mixture used for raising fresh and dry weight of roots. Also, such mixture was superior in increasing survival rate %. Meantime, it raised pigments content in the leaves (chlorophyll a, b, total chlorophylls and carotenoids). Results showed also the prevalence of using division parts in propagation, which to improvise the above mentioned morphological traits studied in both seasons. Meanwhile, using tip cuttings in propagation proved its mastery in raising survival rate %, whereas using mid cuttings was the best in rising chlorophyll (b) in the leaves in both seasons. Also, using either mid cuttings or division parts was the best in raising total chlorophyll in the leaves. Using tip cuttings or division parts was the best in increasing carotenoids content in leaves in the first season, whereas using either mid cuttings or division plants was the best for raising the same content in the second one.<br /> From the aforementioned results, it could be recommended to use division parts in propagation and using the mixture of sand + compost 15% in pots for obtaining the best characters of <em>Russelia equisetiformis </em>plant under local conditions. Moreover, a protocol of micropropagation of <em>Russelia equisetiformis </em>was established using nodal explants taken from the main shoot and lateral branches. Explants were sterilized using 0.1% (w/v) mercuric chloride for 7 and 10 min for the main shoot and lateral branches explants, respectively as 100% of explants were surface decontaminated, survived and stimulated to form shoots. Data of the multiplication stage showed that the highest survival percentages were recorded when explants were cultured on MS medium supplemented with BAP at 0.25 or 0.50 mg/l or kin at 0.25 mg/l which recorded 97.22, 93.06 and 94.44%, respectively. The significantly highest number of the formed shootlets/explant (3.05) was obtained when medium was supplemented with 0.75 mg/l BAP during the third subculture. The significantly highest value of shootlet length (4.72 cm) was recorded when 0.25 mg/l of kin was used during the second subculture. The most suitable treatment for rooting stage was ¾ MS strength which recorded 100% rooting, 8.86 roots/plantlet with 4.56 cm root length. The <em>in vitro </em>propagated plantlets (microshoots with well-developed roots) were hardened in greenhouse with 82% survival rate.<br /> From the above mentioned results, it could be concluded that using tissue culture in propagation is considered the best economic way for the propagation of the<em>Russelia equisetiformis </em>plant comparing with that gained from the other propagation method used. Additionally, it could be mentioned that the profitability rate % of the production by the way of tissue culture method only 780.73% comparing with 338.2% terminal cutting.https://sjfop.journals.ekb.eg/article_12819_c613b4892a312c8ec4ef18da4d085c6d.pdfScientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645120180331INFLUENCE OF SOME PRESERVATIVE SOLUTIONS ON VASE LIFE AND POSTHARVEST QUALITIES OF LIMONIUM CUT FLOWERS891031282010.21608/sjfop.2018.12820ENE. A.KhellaOrnamental Plants & Landscape Gardening Res. Dept., Hort. Res. Inst., Agric. Res. Cent., Giza, EgyptA. M.Abd-ElMoniemOrnamental Plants & Landscape Gardening Res. Dept., Hort. Res. Inst., Agric. Res. Cent., Giza, EgyptA. M.B.MohamedDepartment of Plant Pathology Res., ARC, Giza, EgyptJournal Article20180225<span>The present study was conducted at the Postharvest Lab. of Ornamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., Giza, Egypt in March of 2016 and 2017 seasons, to examine the effect of some pulsing solutions on improving the quality of limonium cut flowers.Limoniumcut flowerswere pulsedin silver thiosulfate (STS) at 500 mg/l for 1/4 and 1/2 h, silver nitrate (AgNO<sub>3</sub>) at 500 mg/l for 1/4 and 1/2 h, AgNO<sub>3</sub> at one g/l for 1/4 and 1/2 h, sodium benzoate at 250 mg/l for 12 and 24 h, sodium benzoate at 500 mg/l for 12 and 24 h, daminozide at 25 mg/l for 12 and 24 h and daminozide at 50 mg/l for 12 and 24 h followed by transferring to a holding solution 8-hydroxyqunoline sulphate (HQS) at 200 mg/l + sucrose (20 g/l). Distilled water was used as a control treatment. The obtained results indicated that treatment of <em>Limonium sinuatum</em> cv. Girlie Wings cut flowers treated by STS at 500 mg/l for 1/2 h enhanced water uptake, relative fresh weight, dry weight percentage of cut flowers, floret opening percentage, pigments content, total carbohydrate percentage and total phenols as well as inhibiting growth of all isolated microorganisms and improved quality of flowers, followed by cut flowers treated with STS at 500 mg/l for 1/4 h then AgNO<sub>3</sub> 500 mg/l for 1/2 h. </span>https://sjfop.journals.ekb.eg/article_12820_11c5996047cf1fcf6139b1ba59657cad.pdf