Scientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645320180901IN VITRO CULTURE AND STUDYING THE CHEMICAL COMPOSITION OF THE ESSENTIAL OILS EXTRACTED FROM THREE SAMPLES OF ERIOCEPHALUS AFRICANUS L. PLANT IN EGYPT2192312421210.21608/sjfop.2018.24212ENT.A.D.MohamedOrnamental Horticulture Dept., Fac. Agric., Cairo Univ., EgyptA.M.A.HabibOrnamental Horticulture Dept., Fac. Agric., Cairo Univ., EgyptM.M.EL-ZefzafyMedicinal Plants (Plant Tissue Culture) Dept., National Organization for Drug Control and Research (NODCAR), Giza, EgyptA.I.E.SolimanMedicinal Plants (Plant Tissue Culture) Dept., National Organization for Drug Control and Research (NODCAR), Giza, EgyptJournal Article20180902The present study aimed to establish new protocol for propagation via tissue culture techniques to observe the effect of plant growth regulators especially cytokinins, gibberellic acid and auxins with different concentrations on <em>in vitro</em> growth of <em>Eriocephalus africanus</em> L. for improving the potentiality of regeneration and secondary metabolites production and identification of the main active constituents of volatile oil by GC/MS. The results showed that, the best sterilization treatment was the shoot tip explants rinsed in a solution of clorox at 15% for 15 min was gave the highest values for survival percentage and plant strength 100% and 4.58, respectively also B<sub>5 </sub>medium at full strength gave the best results in the both growth measurements. BAP at 2.00 mg/l recorded the highest values in survival percentage (93.33%), shootlet number/cluster (16.50) and shootlet strength (4.50), respectively. Using the high level from GA<sub>3</sub> (4.00 mg/l) in medium was more effective in the elongation of shootlets. In rooting stage B<sub>5</sub> medium supplemented with 0.50 mg/l IBA and 0.15% active charcoal was more effective for increasing root number/explant to 8.67 and root length to 5.78 cm. The chemical analysis for the volatile oils extracted from three samples (field, <em>in vivo</em> and <em>in vitro</em>) of <em>Eriocephalus africanus</em> L. by using GC/MS confirmed that, the total number of the constituents identified ranged from 30-34 compounds representing 79.67-99.41% of the total oil contents. Artemisia ketone (17.10-30.62%), bicyclogermacrene (4.14-15.56%), globulol (2.17-8.30%), allo-aromadendrene epoxide (0.51-9.00%), caryophyllene oxide (2.85-6.76%) and α-pinene (3.10-4.25%) were the main compounds.Scientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645320180901INVESTIGATION OF MORPHOLOGICAL AND ANATOMICAL CHANGES IN CATHARANTHUS ROSEUS (L.) G. DON DUE TO COLCHICINE INDUCED POLYPLOIDY2332432421610.21608/sjfop.2018.24216ENAwadShalaMedicinal and Aromatic Plants Research Department, Sakha Horticulture Research Station, Horticulture Research Institute. Agricultural Research center.Z.DengDepartment of Environmental Horticulture, Gulf Coast Research and Education Center,
University of Florida, USA.Journal Article20180909<em>Catharanthus roseus </em>(L.) G. Don is valuable medicinal plant grown as an ornamental plant. The study was carried out at Gulf Coast Research and Education Center, University of Florida, USA. to evaluate the morphological and anatomical changes from induced polyploidy in periwinkle for this purpose colchicine was applied at four different concentrations 0.0, 0.05, 0.1 and 0.2% for seedling treatment. The results showed that raising colchicine doses caused an increasing of survival percentage and tetraploid plants number. Polyploids showed an increment in leaf thickness, stomatal size, pollen diameter and pollen viability compared to diploid plants. Flow cytometry verified the existence of tetraploids and mixoploids from various colchicine-treated plants especially 0.2 which was found efficient on inducing polyploidy in the plants followed by 0.1% as compared to other concentration under study.Scientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645320180901RESPONSE OF JATROPHA CURCAS L. SHRUBS TO COMPLETE (NPK) FERTILIZER, BIO AND ORGANIC FERTILIZERS UNDER SANDY SOIL CONDITIONS2452592660410.21608/sjfop.2018.26604ENE. A.A.El-AzzonyMedicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Giza, Egypt.E. E.A.M.El-MogyMedicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Giza, Egypt.N. S.A.El-ShaipMedicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Giza, Egypt.Journal Article20180913The present work was carried out during the two successive seasons of 2015 and 2016 at the Experimental Farm of El-Quassassin Horticultural Research Station, Ismailia Governorate, Egypt, to study the effect of complete NPK fertilization rates (control, 50, 75 and 100% from the recommended dose), bio and organic fertilizers (control, EM<sub>1</sub> applied as the rate of 100 ml/shrub and active dry yeast with the concentration of 4 g/las a rate of 2 liters/shrub) and the interaction treatments between them on the growth, (fruits and seeds yields), fixed oil content and its components in seeds of Jatropha (<em>Jatropha curcas</em>) shrubs.The results showed that, the best vegetative growth parameters (increasing percentage of shrub height, number of branches and canopy width/shrub), number and weight of fruits (kg)/shrub, number and weight of seeds (kg)/shrub, the yield of fruits and seeds (ton)/fed, fixed oil percentage, weight of fixed oil (g)/shrub and yield of fixed oil (kg)/fed were recorded from the interaction treatment of 75% complete NPK fertilization + EM<sub>1</sub>. The analysis of fixed oil of Jatropha shrub showed that, the treatment of 50% NPK gave the best palmitic acid percentage and the treatment of 50% NPK + EM<sub>1</sub> recorded maximum percentages of stearic and oleic acids, while the treatment of 100% NPK + EM<sub>1</sub> gave the highest percentage of linoleic acid.Scientific Society for Flowers and Ornamental PlantsScientific Journal of Flowers and Ornamental Plants2356-78645320180901RESPONSE OF TUBEROSE PLANTS (POLIANTHES TUBEROSA L.) TO CHEMICAL AND BIO FERTILIZATION AND THEIR EFFECT ON VEGETATIVE GROWTH, FLOWERING AND CHEMICAL COMPOSITION UNDER SANDY SOIL CONDITIONS2612733146710.21608/sjfop.2018.31467ENK.E.AttiaMedicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Egypt.Naglaa F.S.ElbohyOrnamental Plants and Landscape Gardening Res. Dept., Hort. Res. Inst., ARC, Egypt.Nahla A.M.AshourMedicinal and Aromatic Plants Res. Dept., Hort. Res. Inst., ARC, Egypt.Journal Article20180916A field experiment was carried out at the Experimental Farm of El-Kassasin Horticultural Research Station, Agricultural Research Center, Ismailia Governorate, Egypt, to study the effect of partial substitution of chemical nitrogen fertilizer with biofertilizer containing two strains of nitrogen-fixing (<em>Azospirillum lipoferum, Azotobacter chrococcoum</em>), on vegetative growth, flowering and chemical composition of tuberose plants (<em>Polianthes tuberosa</em> L.) under sandy soil conditions to rationalize the use of mineral fertilizers and reduce pollution of the environment in order to increase the quantity and improvement quality of the product on the tuberose plants during the two successive seasons of 2017 and 2018. This experiment included seven treatments with three replications, which were the combinations between two of mineral nitrogen rates (50% and 75%) and three bio-fertilizers of nitrogen-fixing bacteria (<em>Azospirillum lipoferum</em> (S), <em>Azotobacter chrococcoum</em> (Z) and <em>Azospirillum lipoferum</em> + <em>Azotobacter chrococcoum</em> (S*Z) treatments in addition to the control which was 100% N. The treatments were arranged in Randomized Complete Block Design. The results showed that fertilizing tuberose plants with mineral nitrogen at 100% of the recommended dose recorded the highest values for plant height, number of leaves/plant and number of florets/spike. while fertilization with mineral nitrogen at 75% plus the dual inoculation with <em>Azospirillum</em> + <em>Azotobacter</em> recorded the highest values for fresh and dry weights of leaves (g)/plant, fresh and dry weights of root (g)/plant, fresh and dry weights of spike (g)/plant, floret diameter (cm), spike length (cm), longevity of tuberose cut flower spikes (days), in addition to leaves content of N, P, K, total chlorophyll a, b and carbohydrates %, as well as essential oil percentage in flowers without significant difference between them and control in most cases in both seasons.