THE EFFECT OF PLANT GROWTH REGULATORS ON SHOOT FORMATION FROM CORM EXPLANT OF HEDYCHIUM CORONARIUM

Document Type : Original Research Article

Authors

1 Medicinal Plants Dept. (Plant Tissue Culture), National Organization for Drug Control and Research (NODCAR), Giza, Egypt

2 Horticulture Research Institute, Agricultural Research Center, Egypt

Abstract

This study was conducted for a micropropagation of Hedychium coronarium,an important medicinal herb, through sprouted rhizome buds. Rhizome buds were cultured on two different media; Murashige and Skoog medium (MS) and Gamborg medium (B5) without any growth regulators during the establishment stage. B5 medium gave a higher percentage of shoot formation 97.30% corresponding in shoot number and length comparing with MS medium. For shoot proliferation the micro shoots were placed on the B5 medium with two different cytokinins, kinitein (kin) and benzyl adenine (BA) at the concentrations of 0.0, 0.5, 1.0, 2.0 and 4.0 mg/l. Four mg/l of BA led to obtain high value of shoot production. For root formation, the produced shoots were placed on B5 medium plus different concentrations of two types of auxins IBA and IAA at the concentrations of 0.5, 1.0, 2.0 and 3.0 mg/l for each to study the best auxin and concentration which encouraged root formation (number and length). IBA proved a significantly efficient than IAA and encouraged root formation for both root number and root length. IBA at 3.0 mg/l gave the highest average of root number and length. All good rooted plants were cultured into pots containing vermiculite + peatmoss (1:1) in greenhouse, with 82% of survival percentage.

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