IN VITRO SELECTION AND CHARACTERIZATION OF SALVIA OFFICINALIS SALT-TOLERANT CLONES

Authors

1 Dept. of Horticulture, Fac. of Agric., Minia Univ., Egypt

2 Dept. of Horticulture, Fac. of Agric., South Valley Univ., Egypt

Abstract

Salvia officinalis L. is a widest-spread attractive plant in different pharmacopoeias throughout the world. Shoot clumps were regenerated from leaf explants on MS medium enriched with 1 mg/l IAA + 1.5 mg/l TDZ. A 12-week old shoot clumps were subcultured on the same medium supplemented with 100 mM of NaCl. After 12 weeks 4 shoots were recovered from 60 cultured shoot clumps. These shoots were elongated and individually cloned on MS medium enriched with 1 mg/l IAA. Single nodes of the recovered clone, as well as, the control plants were cultured using a single node explants on MS medium enriched with 1 mg/l IAA and 0 or 100 mM NaCl. After a month of growth on this medium statistical analysis showedsignificant difference among the selected clones and the control plants on the base of the ability to develop new growth, plant height, shoot and root fresh and dry weights, as well as, chlorophyll content. Moreover, there were significant differences among the selected colons on proline and total soluble protein content. Also, some regenerated clone showed higher activity of the free radical scavenging enzymes; catalase, peroxidase, and ascorbate peroxidase under salt stress. Therefore, somaclonal variation was used to select a drought tolerant S. officinals clone. Moreover, some physiological mechanisms that are crucial for salt stress tolerance were underlined.

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